This week’s case comes from a 35-year-old patient who presented with:
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Fatigue and mild shortness of breath
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A history of intermittent anaemia
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Mild splenomegaly on examination
The laboratory results showed:
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WBC: 7.4 × 10⁹/L (normal)
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RBC: 5.69 × 10¹²/L (slightly raised)
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Hb: 102 g/L (low)
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HCT: 0.335 (low)
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MCV: 59 fL (low)
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MCH: 18.0 pg (low)
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RDW: 24.6 % (raised)
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Platelets: 157 × 10⁹/L (normal)
Special investigations:
No evidence of common haemoglobin variants (HbS, HbC) or β-thalassaemia trait. The sample was referred for further molecular analysis.


The blood film images above are stained using a special stain. What is the stain used and what is the diagnosis?
